Please see the Methylamp DNA Capture Kit if you are working with purified DNA as the starting material. The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from cells. In the assay an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR and southern blot as well as DNA microarray. WHY CHOOSE THE EPIQUIK™ MeDIP KIT‌

The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian cells. Particularly this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted sheared and added into the microwell immobilized with the antibody. DNA is released from the antibdy-DNA complex and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

CP1 (Wash Buffer)CP2 (Antibody Buffer)CP3A (Pre-Lysis Buffer)CP3B (Lysis Buffer)CP4 (ChIP Dilution Buffer)CP5 (DNA Release Buffer)CP6 (Reverse Buffer)CP7 (Binding Buffer)CP8 (Elution Buffer)Normal Mouse IgG (1 mg/ml)*Anti-5-Methylcytosine (1 mg/ml)*Proteinase K (10 mg/ml)*br />8-Well Assay Strips (with Frame)8-Well Strip CapsF-Spin ColumnF-Collection TubeUser Guide* For maximum recovery of the products centrifuge the original vial after thawing prior to opening the cap.

1. What is the difference between the Methylamp Methylated DNA Capture Kit (#P-1015) and the EpiQuik Methylated DNA Immunoprecipitation Kit (#P-2019)‌The Methylamp Methylated DNA Capture Kit is for purified DNA as the starting materials while the EpiQuik Methylated DNA Immunoprecipitation Kit is for nuclear extract as the starting materials. Also kit P-2019 is specifically designed for immunoprecipitating meDNA fraction directly from cells. The main advantage of P-2019/2020 is that no pre-isolation/purification of DNA is required if mammalian cells/tissues are used. 2. If I am using kit P-2019 starting with purified DNA rather than cells do I have to modify any steps in the protocol‌ When using kit P-2019 purified DNA cannot be used as starting material. The washing buffer lysis buffer and reversing buffer in the kit are not suitable for purified DNA. 3. How can I enrich 200 ng of mDNA as required for a microarray‌Pool the IPed mDNA from 10-20 wells and then purify the mDNA with 5-10 columns (2 wells/each column). It is also useful to generate sufficient DNA for a microarray by amplifying IP’ed mDNA using whole genome amplification (WGA). 4. On page 10 of the user guide does the "input" vial need to add 150 µl CP7 as the samples do‌Yes CP7 should also be added into the "input" vial for DNA binding to the column.

Batchel . et. al. (May 2010). Methylation determines fibroblast activation and fibrogenesis in the kidney. Nature Medicine. 16(5): 544-50. PubMed Abstract